1/14/2024 0 Comments Johny 7 oma![]() These days, toy guns aren't particularly en vogue, and little boys turn instead to Indiana Jones whips and light sabres when attacking their annoying little sisters. To enhance the experience you could purchase a helmet and a pair of walkie-talkies. And if all those functions weren't enough for you, Deluxe Reading (who also produced Johnny Lightning Cars and the Crime-buster Gun) went on to produce a range of accessories under the Topper Toys umbrella for the Johnny Seven OMA, including spare ammunition to replace all those bullets you lost over the next-door neighbour's fence. Now seen as a collector's item, the Johnny Seven OMA has been known to fetch over £300 fully armed on eBay! The stock could be removed to shorten the weapon while in the Tommy Gun mode. Johnny Seven also featured a bipod that provided stability for the various rockets and grenade. The Grenade Launcher was on top of the gun. ![]() Three different rockets (The green Anti Tank, Anti Bunker and the red Armour Piercing fired via spring-action on the main barrel. The Rifle function shot 12 white bullets one at a time via a bolt action spring mechanism through the silver barrel. All of the firing mechanisms were attached to the main rifle - the pistol (based on a design for a real 1950s gun, no less!) inserted from the bottom to provide the rifle grip (the pistol also held caps for authentic firing sounds). It also came with a raft of seven functions (hence the name Johnny Seven). In fact, it wasn't far off full-size! When fully assembled it weighed 4lb and measured an impressive 3ft long, at least. The Johnny Seven OMA (not to be confused with the far simpler Johnny Eagle Rifle of the 70s) had to be the biggest toy machine gun you could get back then, and more than enough to make you throw down your spud gun. And it seems that 1964 was the year most parents decided to abandon their morals, for the Johnny Seven OMA (One Man Army) Gun was the best-selling boys' toy that Christmas. And there comes a point in every parent's life when they have to decide whether it's morally right or wrong to go buy their kid a toy gun. coli, produced 2.7 mg/liter (8.9 μM) 7-OMA upon supplementation with 500 μM p-coumaric acid in 24 h, whereas the strain expressing only the flavanone modification enzymes yielded 30 mg/liter (99.2 μM) 7-OMA from 500 μM NRN in 24 h.There comes a point in every little boy's life when he decides, in the style of John Rambo, he wants (read has to have) a toy gun. Overexpression of the genes for flavanone biosynthesis and modification pathways, along with malonyl-CoA overproduction in E. Dihydrokaempferol (DHK) (aromadendrin) and sakuranetin (SKN) were produced as intermediate products. Thus, produced NRN was hydroxylated at position 3 by flavanone-3-hydroxylase from Arabidopsis thaliana, which was further methylated at position 7 to produce 7-OMA in the presence of 7-O-methyltransferase from Streptomyces avermitilis. In order to increase the availability of malonyl-CoA, a critical precursor of 7-OMA, genes for the acyl-CoA carboxylase α and β subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinica were also introduced. Primarily, naringenin (NRN) (flavanone) synthesis was achieved by feeding p-coumaric acid and reconstructing the plant biosynthetic pathway by introducing the following structural genes: 4-coumarate-coenzyme A (CoA) ligase from Petroselinum crispum, chalcone synthase from Petunia hybrida, and chalcone isomerase from Medicago sativa. Here, we report the generation of 7-OMA from its precursor, p-coumaric acid, in E. To produce such valuable natural flavonoids in large quantity, an Escherichia coli cell factory has been developed to employ various plant biosynthetic pathways. 7-O-Methyl aromadendrin (7-OMA) is an aglycone moiety of one of the important flavonoid-glycosides found in several plants, such as Populus alba and Eucalyptus maculata, with various medicinal applications.
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